To calculate the peak area, multiply the height of the peak (h) by the full width at half maximum (w₁/₂). This gives an approximation of the area under the peak, which is useful for quantifying signal strength or substance amount.
The Peak Area Calculator is an important tool in the world of chemistry, especially in chromatography. It helps us measure and understand the features of peaks in chromatograms. Let’s talk about the formula, how to use it, and why it matters in simple terms.
Formula:
| Variable | Meaning |
|---|---|
| PA | Peak Area |
| h | Height of the peak |
| w₁/₂ | Full width at half maximum (FWHM) |
Solved Calculations
Example 1:
Given Values:
- h = 20 units
- w₁/₂ = 5 units
| Calculation | Instructions |
|---|---|
| PA = 20 × 5 | Multiply the height of the peak by the full width at half maximum. |
| PA = 100 | Perform the multiplication. |
Answer: PA = 100 units²
Example 2:
Given Values:
- h = 15 units
- w₁/₂ = 8 units
| Calculation | Instructions |
|---|---|
| PA = 15 × 8 | Multiply the height of the peak by the full width at half maximum. |
| PA = 120 | Perform the multiplication. |
Answer: PA = 120 units²
What is Peak Area Calculator ?
In chromatography, such as HPLC and GC-MS, determining the peak area is essential for quantifying the concentration of analytes in a sample. The peak area represents the total response from the analyte as it passes through the detector, and it is often preferred over peak height for more accurate results. T
It allows you to input the relevant data, such as retention time and signal intensity, to quickly calculate the area under the peak. This is especially helpful when calculating the area under the curve (AUC) in HPLC, which directly correlates with the concentration of the compound being analyzed.
Aside from its application in chromatography, the calculator can also be used for other types of area measurements, such as irregular shapes or land area calculations. Whether you’re calculating the peak area in square meters or square feet, the tool provides accurate and fast results.
The calculator is also equipped to handle the peak area ratio and other complex formulas like the peak purity formula in HPLC, making it a versatile resource for laboratory professionals and researchers working in analytical chemistry or biochemistry.
