To calculate the extinction coefficient, divide absorbance (A) by the product of molar concentration (c) and path length (m).
The Extinction Coefficient Calculator is an essential tool in biochemistry and chemistry for calculating the molar extinction coefficient, which quantifies how strongly a substance absorbs light at a specific wavelength.
This value is crucial for determining protein concentrations, DNA and RNA quantifications, and peptide or oligonucleotide analysis.
Formula
Contents
E = A / (mc)
| Variable | Description |
|---|---|
| E | Extinction coefficient (in L·mol⁻¹·cm⁻¹) |
| A | Absorbance at a specific wavelength |
| m | Path length (in cm) |
| c | Molar concentration of the substance (in mol/L) |
Solved Calculations
Example 1:
| Input | Value |
|---|---|
| Absorbance (A) | 1.2 |
| Path Length (m) | 1 cm |
| Concentration (c) | 0.01 mol/L |
| Formula Used | E = 1.2 / (1 ∗ 0.01) |
| Extinction Coefficient (E) | 120 L·mol⁻¹·cm⁻¹ |
Example 2:
| Input | Value |
|---|---|
| Absorbance (A) | 0.6 |
| Path Length (m) | 2 cm |
| Concentration (c) | 0.005 mol/L |
| Formula Used | E = 0.6 / (2 ∗ 0.005) |
| Extinction Coefficient (E) | 60 L·mol⁻¹·cm⁻¹ |
What is the Extinction Coefficient Calculator?
The Extinction Coefficient Calculator is an essential tool. You can use it for determining the light absorption capacity of substances like proteins, DNA, RNA, or peptides at specific wavelengths. This value, expressed as the extinction coefficient, helps researchers analyze concentrations and properties of biological or chemical samples using Beer’s Law.
This calculator supports various applications, such as protein extinction coefficient calculations at 280 nm (A280), estimating coefficients for oligonucleotides, or determining extinction values for specific sequences.
It is particularly useful for molar extinction coefficient calculations, allowing precise measurements in fields like biochemistry, molecular biology, and spectroscopy. Tools like the oligo extinction coefficient calculator and protein extinction coefficient calculator streamline this process, enabling fast and reliable results.
It also answers key queries, such as how to calculate molar extinction coefficients, how to find extinction coefficients from sequences, or determining coefficients for DNA and RNA molecules.
With applications in peptide analysis, protein concentration estimations, and chemical research, this tool is invaluable for both academic and professional use.
Final Words:
Ultimately, the Extinction Coefficient Calculator simplifies complex calculations, ensuring accurate and efficient analysis of molecular absorption properties. It is a must-have for researchers seeking precision and reliability in their work.
