Multiply the initial concentration by the dilution factor and divide by the final volume to determine bacterial concentration.
The Bacterial Concentration Calculator is a valuable tool for microbiologists and laboratory professionals. It calculates the concentration of bacteria in a sample based on colony-forming units (CFU), dilution factors, and sample volume. This calculation is essential for applications in microbial studies, pharmaceutical research, and food safety.
Formula
BC = C ∗ DF / VC
| Variable | Definition |
|---|---|
| BC | Bacterial Concentration (CFU/mL) |
| C | Colony-Forming Units (CFU) |
| DF | Dilution Factor |
| VC | Volume of Culture (mL) |
Solved Calculations
Example 1: A sample has 100 CFU with a dilution factor of 10 and a volume of 1 mL.
| Variable | Value |
|---|---|
| Colony-Forming Units (C) | 100 |
| Dilution Factor (DF) | 10 |
| Volume of Culture (VC) | 1 mL |
| Bacterial Concentration (BC) | 1,000 CFU/mL |
Example 2: A sample contains 200 CFU with a dilution factor of 5 and a volume of 2 mL.
| Variable | Value |
|---|---|
| Colony-Forming Units (C) | 200 |
| Dilution Factor (DF) | 5 |
| Volume of Culture (VC) | 2 mL |
| Bacterial Concentration (BC) | 500 CFU/mL |
What is Bacterial Concentration Calculator?
The Bacterial Concentration Calculator is a precise tool used to determine the concentration of bacterial cells in a sample, often expressed as colony-forming units (CFU) per milliliter (mL).
It is commonly used in microbiology to quantify bacterial cultures from dilution series or optical density measurements.
For example, this calculator can estimate CFU/mL using the sample’s dilution factor and plate count. Additionally, advanced versions like the CFU calculator from OD600 or the bacterial concentration calculator ultra allow users to calculate bacterial concentration from optical density values or other experimental data.
This tool is essential for microbiologists, researchers, and laboratory technicians who need to standardize bacterial cultures or assess microbial loads in various samples.
Final Words:
In summary, the Bacterial Concentration Calculator simplifies bacterial quantification by providing accurate and reliable results. It is indispensable for scientific experiments, quality control, and microbial studies.
